Answer:
Following heat shock or electroporation, transformed cells are cultured in antibiotic-free liquid medium for a short period to allow expression of antibiotic resistance gene(s) from the acquired plasmid to begin (Figure 5). This step improves cell viability and cloning efficiency.
Final answer:
Cells need time to recover after a heat shock to refold misfolded proteins with the help of heat shock proteins (HSP), ensuring proper cell function and survival.
Explanation:
Cells need time to recover after a heat shock because the increase in temperature can damage proteins, causing them to misfold. Heat shock proteins (HSP) are a class of chaperones that aid in refolding these damaged proteins. When cells are exposed to high temperatures, they activate these heat shock proteins by releasing them from the NR/HSP complex and increasing the transcription of HSP genes. The recovery period allows these proteins to repair and refold misfolded proteins, ensuring that the cell maintains proper function and avoids the accumulation of damaged proteins which could lead to cell death.
In the wake of a heat shock, cells enter a lag period where they may need to synthesize RNA, enzymes, and other essential metabolites to adapt to environmental changes and repair any damage incurred. This is crucial for cell survival as continued stress without the chance to recover can result in cell death.
Which observations would be most helpful in identifying this spider? Check all that apply.
It is shiny black.
It has a red spot on its abdomen.
It has eight legs.
It has a round, plump abdomen.
It has a hard exoskeleton.
Answer:
A, B, D
Explanation:
Important observations for spider identification include a shiny black color, a red spot on the abdomen, eight legs, a plump abdomen, and a hard exoskeleton. A red spot, especially shaped like an hourglass, often indicates a black widow spider.
To identify a spider, certain observations are most helpful. Observations such as a shiny black color, a red spot on the abdomen, the presence of eight legs, a round plump abdomen, and a hard exoskeleton would all contribute significantly to spider identification. Notably, the red spot on the abdomen is distinctive of the black widow spider, which is known for its venom that can be toxic to humans. All spiders, as arachnids, possess certain characteristics such as eight legs, which distinguishes them from insects. Distinguishing features like leg length, hairiness, and relative size of body segments can assist in classifying spiders into groups.
What happens to Earth when it is farthest from the sun?
It moves fastest.
It stops rotating.
It moves slowest.
It stops revolving.
Answer:
slower
Explanation:
winter days shorter farther from the sun causes stuff
Which of the following results in greater genetic variation in offspring?
A. meiosis
B. both mitosis and meiosis
C. mitosis
D. neither mitosis nor meiosis
Answer:
A. meiosis
Explanation:
Meiosis results in greater genetic variation in offspring compared to mitosis due to processes like crossing over and independent assortment. Mitosis produces genetically identical cells, while meiosis combines genetic material from two parents. Meiosis II is similar to mitosis, and fertilization leads to the formation of a diploid cell.
The greater genetic variation in offspring results from meiosis, not from mitosis. Equal segregation of alleles into daughter cells happens during both mitosis and meiosis. However, genetic variation is specifically introduced during meiosis through mechanisms such as crossing over, independent assortment, and the combination of genetic material from two different parents during fertilization. Mitosis produces genetically identical cells, hence it does not lead to genetic variation.
The part of meiosis that is most similar to mitosis is meiosis II, because it is essentially a mitotic division of the haploid cells formed during meiosis I. Furthermore, the event that leads to a diploid cell in a life cycle is fertilization.
The chronological dating that relates geological rock layers to time is called the options: A. Geologic Lime Scale B. Geologic Time Scale C. Geologic Mime Scale
Answer:
B. Geologic time scale.
Explanation:
The geologic time scale is a system of chronological dating that relates geological strata to time. It is used by geologists, paleontologists, and other Earth scientists to describe the timing and relationships of events that have occurred during Earth's history
Answer:
B. Geologic time scale.
Explanation:
In fungi, which is a disadvantage of asexual reproduction as compared to sexual reproduction?
Asexual reproduction requires ideal environmental conditions.
Asexual reproduction results in offspring that are genetically unique.
Asexual reproduction requires the production of spores.
Asexual reproduction results in fewer offspring.
Answer:
A
Explanation:
This relationship between the bee and the flower is an example of _______.
Answer:
Symbiotic Mutualistic relationship
Explanation:
The bees get honey, and the flowers get to reproduce through their pollen being spread by the bees. Both parties benefit.
This is a star less than mass of the main sequence star that has exhausted all of its nuclear fuel and has collapsed to a very small size.
Answer:
White dwarf
Explanation:
These are what stars become when have exhausted their nuclear fuel. When the stars near the end of its nuclear burning stage, they expels most of their outer material thereby creating a planetary nebula in which only the hot core of the star remains.
They are stars which have less than mass of the main sequence star that has exhausted all of its nuclear fuel and has collapsed to a very small size.
6. Which of the following is NOT an environmental benefit of plants?
A. Provide shade
B. Increase erosion
C. Provide cooler temperatures
D. Filter air
Answer:
the answer is increase erosion
Explanation:
Plants do not increase erosion instead they reduce it
The option that is NOT an environmental benefit of plants is B. Increase erosion. Plants provide shade, cooler temperatures, and filter air, but they generally prevent erosion, not increase it.
Explanation:The answer to which of the following is NOT an environmental benefit of plants is B. Increase erosion. Plants have numerous environmental benefits, some of which include:
A. Provide shade - Trees and plants offer shade, which can cool down areas and provide protection from the sun.C. Provide cooler temperatures - Through the process of transpiration, plants release water into the atmosphere, which helps cool the environment.D. Filter air - Plants absorb carbon dioxide and release oxygen, and they can also filter out pollutants from the air.However, plants generally do not increase erosion; in fact, they often prevent erosion by stabilizing the soil with their root systems. Therefore, option B, Increase erosion, is the correct answer as it is not an environmental benefit of plants.
Black truffles are a unique type of fungi that are highly prized by chefs and food enthusiasts throughout the world. Which characteristic is seen in truffles but not in common mushrooms?
lack a reproductive phase
contains hyphae
has spores contained in ascus
has spores contained in basidia
Answer:
has spores contained in ascus
Explanation:
Ascospores with honeycomb surface decoration may be seen under a microscope; each sac or ascus frequently contains two of these spherical spores. The correct answer is c) has spores contained in the ascus.
What is a black truffle?A fruiting fungus that is tasty is the black truffle. They are a favorite food in France and are highly well-liked there. In order to create some of the most beautiful gourmets, they are both grown and harvested from natural sources. They are one of the most valued products for commerce and are often gathered in the autumn.
Fungi, which include yeasts, molds, and mushrooms, are eukaryotic organisms that play a major role in environmental decomposition. Ascomycota is the phylum in which the flavorful edible fungus known as the black truffle resides. They are expensive and are often found in southern Europe. The ascus, a sexual spore-bearing cell containing ascospores, is the primary characteristic of the Ascomycota. Black truffles have a spherical form, a skin color of black-brown, and little cusps that resemble pyramids.
Numerous asci (spore-bearing cells) and tightly woven hyphae (a long filament-like structure) are present. Each ascus includes spores known as ascopores, which are unique to fungi included under the ascomycetes order. The phylum of Basidiomycota includes mushrooms. Additionally made of hyphae, they reproduce sexually by forming special cells called basidia, which produce spores known as basidiospores.
Therefore the correct answer is c).
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A sample of digestive juice was removed from the stomach of a pig. The juice was
placed in a test tube along with some grains of wheat. A second test tube was set up
containing an equal number of wheat grains, with distilled water rather than
digestive juice. The test tubes were kept for 8 hours at 40 degrees C. After 8 hours,
the grains inside the digestive juice had broken into tiny particles. The grains of
wheat, in the water, were wet but otherwise unchanged.
What is the control group?
test tube with wheat and water
test tube with wheat and digestive juice
the same temperature
the presence or absence of digestive juice
Answer:
The control group is the test tube with wheat and water.
Explanation:
This is because the control group is set up to be compared to the outcome of the test subject influenced by the independent variable, which is this case is the digestive juice. The water and wheat shows the true effect of the digestive juice on breaking down part of a pig's diet, which is wheat.
Which level of organization is pictured?
organelle
cell
tissue
organ
Answer:
organ
Explanation:
Answer:
D: organ
Explanation:
Have a good day :)
Does the law of reflection hold true for mirrors that aren’t flat ?
The law of reflection applies to both flat and curved mirrors, stating that the angle of incidence equals the angle of reflection. Curved mirrors, such as convex and concave mirrors, reflect light in a way that alters the image size, orientation, and whether it's real or virtual. The size of the object does not change the laws of reflection, which can be confirmed through optical experiments.
Explanation:The law of reflection states that the angle of incidence is equal to the angle of reflection, with the incident ray, the reflected ray, and the normal (the line perpendicular to the surface) all lying in the same plane. This law applies to both flat and curved surfaces such as mirrors. When considering a flat mirror, the law of reflection leads to virtual images which are the same size as the object. The image appears as far behind the mirror as the object is in front of it.
In the case of a convex or concave mirror, the reflection still obeys this fundamental law, but the curvature of the mirror causes the reflected rays to diverge or converge, which affects the characteristics of the image. The /size, orientation, and type (virtual or real) of the image can vary/ based on the distance of the object from the mirror and the focal point. For example, in a concave mirror, when an object is closer than the focal point, the image is virtual and enlarged; beyond the focal point but closer than twice the focal length, the image is real and inverted. When the object is further away than twice the focal length, the image is smaller and still inverted.
Regarding the question of whether the /size of the object changes the laws of reflection/, it does not. The laws remain constant, but the size of the object can affect the visibility and the extent of the reflected rays across the mirror's surface, particularly for curved mirrors. In optical experiments, using flashlights and mirrors, the law of reflection can be observed and confirmed, either with a plane mirror producing predictable reflection angles or with curved mirrors that focus light in specific ways, like in flashlights or concert shells.
Which are deuterostomes?
A. flowering plants
B. humans
C. fungi
D. slime molds
Answer:
humans
Explanation:
What does the size and shape of the proteins decide?
A). How fast the protein can Get into the blood
B). How big an organism can grow
C). Function that protein will have in inside the body
D). Weather the organism is a plant or animal
Answer:
the answer is c
Explanation:
Identify the initial event responsible for the new sweet-sensing gene.
Answer:
A mutation
Explanation:
A mutation is a change in the sequence of an organism's DNA. Mutations can be caused by high-energy sources such as radiation or by chemicals in the environment.
How has the presence of the sweet sensing gene increased in the hummingbird population over time?
Hummingbirds evolved from insect-eating ancestors. The genes that detect the savoury flavor of insects underwent changes, making hummingbirds more sensitive to sugars. These new sweet-sensing genes give hummingbirds a preference for high-calorie flower nectar.
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In what two ways do plants keep our climate stable over time?
Answer:
Through transpiration and use of carbon dioxide (green house gas)
Explanation:
Plants help in making the climate stable in following two ways –
a) Temperature offsetting – Plants take in carbon dioxide from atmosphere and thereby reduces atmospheric carbon. Due to reduction in greenhouse gases, the temperature reduces.
b) Moisture regulation – Plants transpire through their leaves. Through transpiration, they release water as vapor and cool down the air. The higher the vegetation , the more is the transpiration.
carbon is present in the atmosphere as
Answer:
co2
Explanation:
Are the cells pictured below plant or animal cells?
a
Plant
b
Animal
Answer:
where is the picture? I can't help you without the picture...
Based on structural components, plant cells have a cell wall, chloroplasts, and a large central vacuole, making them distinct from animal cells, which lack these but contain centrioles and lysosomes. These differences are key in identifying the cell type.
Explanation:Identifying whether cells are plant or animal cells involves comparing their structural components. From the given descriptions, we can differentiate these cells based on key features. Plant cells are characterized by the presence of a cell wall, chloroplasts for photosynthesis, plasmodesmata for cell communication, and a large central vacuole that maintains cell turgidity. Conversely, animal cells are notable for their lack of a cell wall, absence of chloroplasts, and instead contain centrioles and lysosomes.
Plant cells have distinct features that set them apart from animal cells. Plant cells possess organelles like chloroplasts for photosynthesis, a large central vacuole for storage, and a cell wall for structural support. On the other hand, animal cells lack these structures and have centrioles, lysosomes, and centrosomes.
The presence of a cell wall, chloroplasts, and a large central vacuole are characteristic features of plant cells, not found in animal cells. Such structures enable plants to perform photosynthesis, provide structural support and store nutrients, respectively. In contrast, animal cells have unique components like lysosomes for waste disposal and centrioles that play a role in cell division. These differences are critical for their distinct biological functions. Therefore, identifying these components is essential in distinguishing between plant and animal cells.
Alab technician needed to make a wet mount slide of their cheek cells. The tech made
his slide and placed it on the microscope stage. Then he got ready to view his cheek
cells by following these procedures.
1. Looking through the eyepiece, the technician adjusted the diaphragm so field of
vision was lit.
2. The technician looked at the stage from the side and lowered the body tube so the
high power objective was almost touching the slide.
3. Looking into the eyepiece, he very slowly turned the coarse adjustment to bring the
cells into focus.
What suggestion would you make to the technician to CORRECTLY use the microscope
to view his cheek cells?
do not look at the stage when lowering the body tube
The question is incomplete as it does not have the options which are:
A) close the diaphragm
B) start with the low power objective
C) do not look at the stage when lowering the body tube
D) start with the body tube raised and lower it while looking through the eye piece
Answer:
Option-B
Explanation:
In the given question, the technician is trying to observe the cheek cells in the light microscope. The person prepares the wet slide of the sample and tries to observe the sample using the protocol provided in the question.
In these options, the technician is not following the rules to observe the slide in the microscope that always observes the specimen using an objective lens with lower power. Then increase the power of the objective lens to increase the magnification of the sample.
Thus, Option-B is the correct answer.
Huntington’s disease is a condition that causes problems of the brain and muscle coordination in some humans. What would most likely be observed in humans who inherit this genetic disease?
A. A decrease in the efficiency of cellular respiration
B. A change in the production of specific proteins
C. An increase in the speed of nerve cells
D. A change in the cellular membranes of muscles
Answer:
D
Explanation:
Answer: D
Explanation: Cuh yeah
two parents of unknown genotype’s are crossed. All of the offspring that are produced are heterozygous round. What are the genotypes of the parents?
Answer:
RR x rr
Explanation:
To have all heterozygous offspring, both parents must be homozygous so each combination of characters is the same
RR x rr -> Rr, Rr, Rr, Rr
True or False: Work is being done in the photo below.
Answer:
true because her muscles are working and theres movement being done overall in the image.
Explanation:
Answer:
true and false she could be pushing the wall or she could be stretching i highly doubt her little muscles can push through that wall though.
Some species of stick insects have wings, others are wingless. recent research indicates that wings have been gained and lost several times during the evolutionary history of stick insects. for the six species on the evolutionary tree, which hypothesis require a lower total number of wing gains and wing losses?
Answer:
It is a hypothesis that explains the origin of evolutionary reversions.
Explanation:
A phylogenetic tree may indicate that genes required for forming anatomical structures such as wings are not completely lost and they are activated many times during the evolution.
9. What type of cellular respiration requires oxygen?
Answer:
Cellular respiration can occur both aerobically (using oxygen) and aerobically (without oxygen).
Explanation:
During aerobic cellular respiration, glucose reacts with oxygen, forming ATP that can be used by the cell. Carbon dioxide and water are created as by-products.
In cellular respiration, glucose and oxygen react to form ATP. As by-products, water and carbon dioxide are released.
In cellular respiration, glucose and oxygen react to form ATP. As by-products, water and carbon dioxide are released.
The three stages of aerobic cellular respiration are glycolysis (an anaerobic process), the Krebs cycle, and oxidative phosphorylation.
There is no____in Rna.____pairs with adenine instead.
A.guanine.cytosine
B.uracil,thyme
C.adenine,uracil
D.thymine,uracil
Answer:
The answer is D.
Explanation:
There is no Thymine in RNA. Uracil pairs with Adenine instead.
what is the dependent variable in Guinea pigs eat more when it is cold.
Sienna made a chart listing different kinds of mollusks.
Which headings correctly complete the chart?
X: Sea Urchins
Y: Gastropods
Z: Cephalopods
X: Bivalves
Y: Cephalopods
Z: Gastropods
X: Gastropods
Y: Cephalopods
Z: Bivalves
X: Cephalopods
Y: Sea Urchins
Z: Gastropods
Answer:
Im pretty sure its C. But correct me if im wrong
X: Gastropods
Y: Cephalopods
Z: Bivalves
Explanation:
Answer:
The answer is C
Explanation:
what other types of anatomical evidence do scientists use to determine the evolutionary relationships of animals?
Answer:
Evidence of evolutionary relationships include: similarities in body structure, fossils, protein structure, similarities in DNA, and similarities in early development. Know how fossils are formed, named, and how scientists determine fossil age. Most fossils form when organisms die and are buried in sediment.
Explanation:
There are two main groups of flowering plants: monocots and dicots. One of the differences between the groups is the structure of the leaves.
Which statement accurately describes the characteristics of monocot and dicot leaves?
Monocot leaves have branched veins, while dicot leaves have straight veins.
Monocot leaves are like needles, while dicot leaves are flat.
Monocot leaves have straight veins, while dicot leaves have branched veins.
Monocot leaves are flat, while dicot leaves are like needles.
Answer:
its C
Explanation:
Answer:
c
Explanation:
TRUE or FALSE Q:Groundhogs inhabiting a meadow environment drink water and rely on plants, including clover, for food. Both the water and the clover that the groundhogs consume can be described as abiotic and biotic factors.
Answer:
TRUE
Explanation:
OKOKOKOKKOOKOKOK
Answer: yes water does not have life. It has life in it but water is not living. Therefore water is abiotic. Clovers actually respond to stimulus, move and are living. Therefore clovers are abiotic.
Explanation: I explained my answer in my answer