When living yeast cells were placed in congo red dye and examined under the microscope, the yeast cells remained colorless. However, when placed in methylene blue, they became blue. Later, dead yeast cells were placed in congo red dye. These cells turned red. Explain these three observations.

Answers

Answer 1
Well, to be honest, some of that doesn't make sense. We'll take each observation individually. 

1. Living yeast cells were placed in congo red dye, but they remained colorless. 

This makes sense. Congo red freely diffuses from high to low concentration across the cell membrane, without using any energy. The cell then pumps the molecules back out of the cells, moving them from a low concentration inside the cell to a high concentration outside the cells. This causes living yeast cells to remain colorless in congo red dye. 

2. Living yeast cells were placed in methylene blue, and they turned blue. 

This is the one that doesn't make sense. Yeast cells that are living contain an enzyme that decolorises methylene blue, whereas dead cells do not. When cells from a yeast sample are suspended in the dye, it penetrates into all the cells, but is broken down only by the living cells. So you can distinguish between living and dead cells by examining them microscopically: dead cells are stained blue and living cells are unstained. 

3. Dead yeast cells were placed in congo red dye, and they turned red. This one also makes sense. As I said in #1, living yeast cells actively pump congo red back out of themselves. If the cells are dead, this pumping does not occur, and the congo red stains the cells. The same would be true if you placed dead cells in methylene blue. The enzyme that normally breaks down the methylene blue would not be working because the cell would be dead, and the cell would turn blue. I hope i helped :p

Answer 2

Living cells remain colorless because of the presence of an enzyme. They cannot reduce the stain. Whereas, dead cells can reduce the stain and appear red while stained with congo red dye.

What is Staining?

Staining is a technique used to enhance the contrast in samples to visualize the samples clearly. This makes the identification and observation of samples easy.

Living cells have an active dehydrogenase system which reduces methylene blue stain and dead cells are unable to reduce this stain. Thus living cells are colorless under this stain while the dead cells stain blue.

The Congo red stain is used for staining amyloids in cell wall of plants, fungi, and outer membrane of gram negative bacteria. Congo red is a synthetic dye, used for the differential staining of elastic fibers present in the cells of bacteria and plants.

Yeast are observed to be oval (egg shaped) organism under high resolution microscopes. It is also possible to observe the buds present on some of the yeast cells.

Methylene blue stain is used in determination of cell mortality. If methylene blue stain is applied to a sample, a healthy cell with turn the stain colorless. This is due to the enzymes, which reduce the methylene blue, causing it to lose its color.

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Related Questions

Volcanoes are usually formed as a result of _____.

a convergent boundary, where both plates are continental

a divergent boundary, where at least one of the plates is oceanic

a transform fault boundary, where both plates are oceanic

a convergent boundary, where at least one of the plates is oceanic

The answer is B. a divergent boundary, where at least one of the plates is oceanic

reposting this question because a guy named taskmasters answered this question and it wasnt even correct.

Answers

A convergent boundary, where at least one of the plates is oceanic

what is the the product of pyruvate processing

Answers

Answer:

Acentyl CoA

Explanation:

Apex

The ____________ of a muscle is usually attached to a fixed location.

Answers

i believe it is origin

What is the advantage of having the bacterial dna near the center of the cell?

Answers

Frfghhhhhhyrrrbsjsvdksvdisvdkfvekvrodvdjr rot

Answer:

The DNA of the bacteria is present in a circular form which can be housed inside the irregularly shaped nucleoid without membrane. The nucleoid contains other biomolecules like proteins, enzymes and RNA along with DNA.  

The bacterial DNA is present in the center of the cell as its central location enables it to reach the to all parts of the cell which could facilitate the cell functions easily. Since DNA controls all the functions of the nucleus, therefore, it's central location is necessary to the cell.

A patient is transferred to a nursing home without arrangements for follow-up being discussed with the family or a clinician responsible for follow-up care

Answers

A good example of clinician-patient relationships may be when a patient is transferred to a nursing home without arrangements for follow-up being discussed with the family or a clinician responsible for follow-up care, it shows it was a patient´s decision. 

In the postanesthesia care unit (pacu), the nurse notes that the patient is having difficulty breathing and suspects an upper airway obstruction. what would the nurse do first?

Answers

The nurse should help the patient sit upright. She can hold a pillow tightly if the incision is on the chest or belly. Now slowly the patient can take normal breaths, then a slow deep breath through the mouth. This may be repeated for 10 to 15 times slowly.

What letters do the names of enzymes usually end in?

Answers

Most enzyme names end with "-ase"
Final answer:

Enzymes typically end with the suffix '-ase'. Examples include lactase, amylase, and lipase. This naming convention helps classify and identify the enzymes and their functions.

Explanation:

In biology, the names of enzymes usually end in '-ase'. For example, lactase, amylase, and lipase are all enzymes. This suffix is a convention in the naming of enzymes based on the reaction they catalyze. It is a universally followed nomenclature system in biology that allows for the easy classification and identification of enzymes and their functions.

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Dna is a polymer, which means that is made up of many repeating single units (monomers). what are the monomers called?

Answers

The monomers of nucleic acids, or DNA/RNA are nucleotides.

Which of the following would be the most important adaptation for a desert-dwelling animal to possess?
a.
heat dissipation structures
b.
heat conserving structures
c.
energy conserving behaviors
d.
water conserving behaviors

Answers

i think the answer is D 

The correct answer is option d. water conserving behavior.

The desert biome lacks water, so, the conservation of the water is very important for the organism for the survival in the deserts. The desert dwelling animals are adapted, in such a way, that the water is conserved in the body of the animals. They do not drink water for many days and utilize the water present in the body.  


In the cell shown below, which lettered structure is responsible for the excretion of most cellular waste

Answers

b, which is the cell membrane.

     Well, the answer to this question could be two options, cell wall and lysosomes.

     The cell wall works in a way that this waste, can be anything that won't be useful to the cell, will be thrown out of the cell through the membrane, and this can happen because this cell membrane is made out of phospholipids, and they adjust themselves in a way that they can let this waste go through them to get out. This membrane also has transmembrane proteins that could help this process of exocytosis.

       The second part, the lysosomes, work in a way that the cell waste goes inside this lysosome, and inside of it, there are multiple different kinds of enzymes that can turn this waste into little molecules that can be used by the cell instead of just throwing it away.

what are some of the health issues that clones may experience?

Answers

Clones cannot produce extra antibodies, cannot alter it's antibodies to protect it from another disease, and these result in the clone being a infestation of germs & bacterias that can cause harm to other people.

hope this helps

Two parents produce offspring that share their characteristics through ____________ p\reproduction

Answers

sexual reproduction...

A 65-year-old patient reports night sweats and weight loss. the complete blood count report shows that she has lymphocytosis. the total white blood cell count is 23,000 microliters. on diagnosis, the bone marrow of the patient is infiltrated with small lymphocytes. what should the nurse suspect based on the information?

Answers

Nurse can suspect Tuberculosis as the patient has night sweats and lymphocytosis. Sputum also need to be cultured to make sure she does not have AIDS.

Areas with little rainfall tend to have basic soils, which have a high pH. Which of the following is an ion known to increase soil pH? A.Sodium B.Oxygen C.Sulfate D.Magnesium

Answers

The answer is D. Magnesium

Magnesium ion increase the soil pH and neutralize the basic soil due to little rainfall. thus, option D is correct.

Who less rainfall affects the pH of soil?

In warm, humid environments, soil pH decreases over time in a process called soil acidification, due to leaching from high amounts of rainfall. In dry climates, however, soil weathering and leaching are less intense and pH can be neutral or alkaline.

Magnesium ion is vital to plant growth and health. It also increase the pH of the soil as it replace hydrogen ion which resulted in increase of acidity of soil.

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"phenylalanine is an amino acid needed by the body and must be ingested through the diet. therefore, this is an example of a(n) _____________ amino acid."

Answers

Answer:

this is an example os thyrosine amino acid

Explanation:

How will the diversity of species differ between early and late succesion areas?

Answers

You could argue that in early succession areas species may have not been adapted or evolved. In Darwin's theory, life began in a shallow, warm pond. If that is the case, species would then venture deeper when their area they are in is suitable and having success

Describe the difference between exocrine glands and endocrine glands. list below an example of each and what hormone they release.

Answers

Endocrine gland is a gland whose secretions (called hormones) are collected by the blood and reach the tissues through the circulation. The hypophysis (pituitary) and the adrenals are examples of endocrine glands. Exocrine gland is a gland whose secretions are released externally through ducts (into the skin, intestinal lumen, mouth, etc.). The sebaceous glands and the salivary glands are examples of exocrine glands.

Which structure in leaves allows the gaseous reactants and products of photosynthesis to move in and out?

Answers

The stoma allows the gaseous reactants and products of photosynthesis to move in and out.
Hope this helps!!

The atomic number is consider a unique atom and element identifier. Describe the associated subatomic particle, and how it can be used to identify the atom.

A) The number of electrons changes in a unique way as the charge on the atom changes.
B) The proton number remains constant through changing the atomic charge, energy, and isotopes.
C) As the atom absorbs photons the way the photons interact in the nucleus gives the atomic number.
D) All isotopes of the an element have the same number of neutrons allowing it to describe the unique atom.

Answers

it is b the number of protons

What happens when you put aluminum foil in a microwave?

Answers

it explodes

hope this helps lol

Final answer:

Placing aluminum foil in a microwave can cause sparks and potentially start a fire due to high-energy radiation causing electrons in the metal to move, leading to rapid heating and damage to the microwave. It's not recommended to put metal in a microwave.

Explanation:

What happens when you put aluminum foil in a microwave? When aluminum foil is placed in a microwave, the electric fields in microwaves cause the electrons in the metal to move, creating currents of electricity. This can cause sparks, or arcing, between points of the foil or between the foil and the sides of the microwave. Additionally, microwaves are high-energy radiation that increases the movement of electrons in materials they interact with, which can lead to the foil heating up rapidly. This can damage the microwave's components or even start a fire. It's important to remember that microwaves heat food by causing water molecules to vibrate, but metals reflect these microwaves, leading to uneven heating and potentially hazardous situations. Therefore, it's not recommended to put metal, such as aluminum foil, in a microwave.

What are two kinds of evidence used by modern taxonomists to classify organisms based on evolutionary relationship?

Answers

The answer is shared characteristics. Hope this helps.

Proteins attract water and hold it within blood vessels, preventing it from freely flowing into the spaces between the cells. this is an example of how protein is used for:​
a. ​maintaining fluid and electrolyte balance.
b. ​building antibodies.
c. ​supporting growth and maintenance.
d. ​maintaining ph balance.
e. ​building enzymes, hormones, and other compounds.

Answers

Supporting growth and maintenance

Why is dna replication defined as semi conservative?

Answers

Because the two new daughter DNA molecules are " Half old" and "Half new". Half the original DNA molecule is saved, or conserved in the daughter molecules.  

How do hydrophilic and hydrophobic relate to the structure of a cell membrane?

Answers

The molecules of lipid comprising the cell membrane have distinct hydrophobic tails and hydrophilic heads. This allows them to collect into layers, and create the cell membrane. 

The tails, which face the inside of the cell membrane, are "hydrophobic," but the heads, which make up the exterior and inner linings, are "hydrophilic".

What is cell membrane?

Cell membrane is defined as the membrane isolates the inside of the cell from the external environment and is present in all cells.

It can also be defined as a biological membrane that keeps all cells' interiors isolated from the outer world and provides protection for it.

Plasma membrane is another name for the cell membrane.

The three main function of cell membrane are:

A cell membrane is made up of lipids and proteins.Transport proteins, glycoproteins, and structural proteins all make up the cell membrane. These preserve the shape and structure of the cell, move materials across the membrane, and communicate between cells.

Cell membrane are both hydrophilic and hydrophobic in nature.

Thus, the tails, which face the inside of the cell membrane, are "hydrophobic," but the heads, which make up the exterior and inner linings, are "hydrophilic".

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If you would like the same label to appear in the first ten cells of column a, you should _____.

Answers

If you're talking about excel then you would select the cell with the label you want and click "Format Painter". Then, you click and drag it down the first 10 and it will automatically apply that same label to all the cells. 

Answer:

Copy and paste by using special function (ALT+E+S+V)

Explanation:

It is easy to work on excel as it allows to increase efficiency by easing the completion of repetitive work.

In this case when same label is to be copied to ten different cell of a column, then one can do the following -

a) put the cursor on the label that is to be copied

b) Press ctrl + C to copy the label

c) Now select the columns in which the labels are to be copied

d) Press ALT + E+ S+V and select format if only format is to be pasted.

True or false amino acids with hydrophobic side chains are often found in the region of an integral membrane protein that spans the membrane.

Answers

True

Further explanation

Amino acids are organic compounds that have a carboxyl (-COOH) and amine (usually -NH2) functional group. This carboxyl group gives acidic properties and the amine group gives basic properties. Protein-forming amino acids will bind to each other with peptide bonds, so that in one dipeptide molecule they contain one peptide bond.

In general, in an amino acid a C atom binds four groups, namely: a carboxyl group, an amine group, a hydrogen atom and a residual group (side chain, -R group). The side chains of the different amino acids (R groups) in amino acids determine the structure, size, electrical charge and water solubility.

There are 2 types of amino acids based on the body's ability to synthesize, namely essential amino acids and non-essential amino acids. Essential amino acids are amino acids that cannot be synthesized in the body, but are obtained from outside, for example through food (lysine, leucine, isoleucine, threonine, methionine, valine, phenylalanine, histidine, and arginine). Non-essential amino acids are amino acids that can be synthesized in the body through an overhaul of other compounds.

Classification of amino acids can be done based on side chains (-R groups) and their solubility in water. Based on the solubility in water divided into hydrophobic and hydrophilic amino acids.

AMINO ACID BIOLOGICAL FUNCTION

- Compiler of proteins, including enzymes.

- The basic framework of a number of important compounds in metabolism

- Binding of important metal ions needed in enzymatic reactions.

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Details

Class: High School

Subject: biology

Keywords : Amino acid, enzymes, carboxyl

Amino acids with hydrophobic side chains are present in the region of an integral membrane protein that spans the membrane. This statement is true.

Further Explanation:

The Fluid Mosaic Model suggests that the plasma membrane is a unique structure with phospholipids, proteins and several carbohydrate molecules. Arrangement of phospholipids provides the membrane fluidity. The transmembrane domain is embedded in the hydrophobic layer, holding the proteins. Their hydrophobic nature is a must for their presence in the hydrophobic environment.Integral membrane proteins (IMPs) are composed of two types of amino acids:

Polar amino acids Non-polar amino acids

The polar part lies close to the hydrophilic part of the lipid bilayer, and the non-polar components lie adjacent to the hydrophobic part. Membrane spanning regions in the plasma membrane is called the transmembrane domain. These domains are made up of some hydrophobic molecules (amino acids) and play a major role in holding the protein in the membrane. Transmembrane domains are present in the hydrophobic layer and hence are similar in nature, providing them stability.  

Learn More:

Learn more about the treatment of eukaryotic cell with a drug https://brainly.com/question/10767798 Learn more about the proteins synthesis in a cell https://brainly.com/question/1420458 Learn more about the exchange of gases by blood cells https://brainly.com/question/1213217

Answer Details:

Grade: High School

Subjects: Biology

Chapter: Cell Membrane

Keywords:

Polar, non-polar, amino acid, hydrophobic, hydrophilic, membrane, stability, nature, molecule, fluid mosaic model, environment, nature.

What is the complementary sequence corresponding to the dna fragment gataca?

Answers

I believe this is called CTAATGT. Hope this helps.

. Which factor does not take a population out of Hardy-Weinberg equilibrium? allele frequency natural selection mutation rate genetic drift

Answers

allele frequency is the answer
Answer;

Allele frequency

Explanation;According to Hardy Weinberg Principle, the gene or allele frequencies in a population will remain stable from generation to generation until acted on by an outside force.Allele frequencies in a population will not change from generation to generation, as far as factors of evolution such as natural selection, mutation, and migration are not taking place.Hardy-Weinberg equilibrium can be disturbed by the forces of evolution which includes, mutations, natural selection, gene flow, non-random mating and genetic drift.

Jamal is asked to write a science essay about molecular forces. He first turns to his science textbook for information. Which is the BEST second source for Jamal’s research?

Answers

There are several sources in which one may turn to in order to establish a well-founded idea, information, and knowledge towards a given topic. From this item, books were collectively identified as the first source for Jamal's science essay.

The second one could be the information found in the internet. These information have lately became very accessible considering that one could directly upload/download information. The tricky part here is to identify which sources in the internet are reliable and which are not. 

Answer:

a scientific journalExplanation:

Which of the below animals is the least related to the others, evolutionarily?

Answers

Please include the animals when you redo this question.
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