Which enzyme(s) will produce a dna fragment that contains the entire vgp gene (shown in red) and has “sticky ends”?

Answers

Answer 1

HindIII and BamHI are the enzymes that will create a DNA fragment that carries the entire vgp gene (shown in red) and has "sticky ends"

Cloning the vgp gene requires an enzyme that has restriction sites on both sides of that gene but not within the gene.  Satisfying that requirement are the two enzymes, namely HindIII and BamHI, while at the same time, creates DNA fragments that contain sticky ends. Sticky ends make it possible for the fragments to combine with the DNA of a cloning vector, such as a plasmid

 

BamH I, on the other hand is a type II restriction endonuclease, having the capacity for recognizing short sequences (6 b.p.) of DNA and specifically cleaving them at a target site. 


Related Questions

Explain how the structure of epithelium and the structure of connective tissue, specifically bone, relate to the function of the tissue

Answers

Epithelial tissue is densely packed cells that used most in the area where protection is needed. This cells mostly used in skin, some with more keratin which will increase their ability to protect. In bone, the main protective layer is calcium mineral which was deposited by osteocytes. Because this bone doesn't really need more epithelial cells. Bone tissue only needs a good vascularization and connective tissue to support it.

I believe the correct answer is they protect the tissue.

Explanation:Epithelium tissue:

The epithelium contains a layer of stacked cells called epithelial cells that are simple and form a tough layer on many surfaces and orifices of the body. They are classified according to their structures i.e. the squamous and stratified epithelium. Squamous epithelial cells line surfaces like the cheeks in the mouth and usually wear away easily in cases of abrasion. Stratified epithelial cells are usually very closely stacked together and are tougher than the squamous epithelial cells. They protect open surfaces like the lips, vagina and the anus where friction is very frequent. The cells can additionally be added with a layer of tough proteins called keratin that cover 100% of the exposed skin, hair and nails. They make the skin waterproof and tough and the cells that have them are usually the stratified epithelial cells e.g. the stratified keratinized epithelial cells.

Connective tissue:

It is one of the types of animal tissues and is very tough since it is used in support and movement where a lot of mechanics occur. It occurs between tissues i.e. linking muscles to bones and they support and also protect the body. The bone is a type of a densely packed connective tissue that is calcified. It contains the osteoblasts that deposit into the bone, the osteocytes that maintains the bone and the osteoclasts that resorb the bone. Despite the bone being tough, it can be classified into the compact and spongy bone. Spongy bone is found in articulation points of the bone and cushions the organ such as the thigh during movement such as jumping up and down. The spongy bone can also be found inside the compact bone and is porous allowing nerves and blood vessels to pass into it. The porous holes are eventually filled by the red or yellow bone marrow. The red bone marrow creates blood cells.

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Level: High School

Subject: Biology

Topic: Support and Movement

What makes sperm cells and egg cells different from almost all other types of body cells.

Answers

They have half as many chromosomes as the rest of the cells (so that when they meet, the chromosomes will be combined to make a different set)

Sperm cells and egg cells are involved in reproductive functions, while somatic cells are involved in growth and maintenance of body.

What is the difference between somatic and sperm cells?

Sperm cells and egg cells are specialized cells that are involved in sexual reproduction and produced through meiosis process. Sperm cells are typically small in size. It has a long tail and a pointed head to penetrate and fertilize the egg cell. Egg cells are much larger in size and rounder in shape. It has a thick outer layer to protects the cell from fertilizing by the sperm cell, while somatic cells are used in growth and maintenance of body.

Sperm cells can survive for a few days outside the male reproductive tract, while egg cells are only viable for a short period of time after ovulation.

The sperm cells and egg cells contain specialized proteins that are involved in the fertilization process and early embryonic development. These proteins and enzymes are synthesized within the sperm cells to help the sperm cells to penetrate the egg cells for fertilization, and successful fusion of the sperm and egg cells. However, somatic cells are not involved in fertilization process.

Therefore, sperm cells and egg cells combine together to form a zygote and create a new life, while somatic cells cannot form zygote.

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What type of smoke screen provides a thick, vertical barrier between enemy and friendly troops?

Answers

The answer is a smoke curtain. It is a dense, vertical development of smoke. It is positioned between friendly and enemy positions to avert or damage enemy ground observation of friendly positions. Since the smoke curtain is not placed directly on friendly troops, it will not hinder friendly operations. Also, it activates in the event of a fire, on condition that a means of diverting smoke away from a preferred location or protecting an area from direct smoke infiltration.

A client has a hiatal hernia. the client is 5 feet 3 inches tall (163 cm) and weighs 160 pounds (72.6 kg). which information should the nurse include when discussing prevention of esophageal reflux?

Answers

The nurse should tell the client to reduce his caloric intake in order to foster weight reduction. This is necessary because, weight reduction decreases the intra-abdominal pressure, thereby reducing the possibility of esophageal reflux. Weight reduction also reduces the amount of fat in the body. Fats usually extending the occurrence of esophagus reflux.

Which principle suggests that inherited traits contributing to reproduction and survival will most likely be passed on to succeeding generations?

Answers

Final answer:

Natural selection is the principle that suggests advantageous inherited traits for reproduction and survival will most likely be passed on to future generations, as individuals with these traits are more likely to survive and reproduce.

Explanation:

The principle that suggests that inherited traits contributing to reproduction and survival will most likely be passed on to succeeding generations is known as natural selection. This concept was famously proposed by Charles Darwin and Alfred Russel Wallace. They recognized through observation and study that individuals within a species exhibit variations in their traits, and some of these traits provide advantages over others in terms of surviving and reproducing in their environment. Consequently, the individuals with advantageous traits are more likely to survive and have more offspring. Over time, these advantageous traits become more prevalent in the population, leading to the evolution of the species.

At the core of natural selection are three key principles:

Most characteristics of organisms are inherited from their parents.

Resources for survival and reproduction are limited, creating competition among individuals.

Offspring vary in their inherited traits, and those variations that confer survival and reproductive advantages tend to be passed down through generations, leading to a change in the population over time. This process is also known as descent with modification.

Ultimately, natural selection is responsible for the adaptive evolution of populations, continuously shaping organisms to be more finely tuned to their environments.

________ refers to any condition affecting the structures or function of the cardiovascular system, and affects more than 70 million americans

Answers

HEART DISEASE refers to any condition affecting ..................... Heart disease is an umbrella word for all the diseases that affect the heart. There are many types of heart disease and they always involve the heart and the blood vessels system. In America, more than 70 million people are suffering from heart disease.

Answer:

Heart disease affects over 70 million people across the world. It is a very serious disease that needs to be made aware of.

Explanation:

What is the maximum number of hours that food can be held in the food danger zone?

Answers

To answer this question, let us first mention what is food danger zone. Well, this refers to the temperature range in which bacteria grows faster in the food. The range is defined as 30 to 140 degree Fahrenheit or can be converted into 4 to 60 degree Celsius. Going back to the question, the maximum number of hours that a food can be held in the food danger zone is only 2 hours. Beyond this 2  hours, the food is not safe to be eaten and the person who dares to eat this food might get some illness and worst might experienced food poisoning.

The maximum number of hours that food can be held in the food danger is zone is 2 hours. Beyond this, food is suspected to have high amounts of bacteria that may cause illness and is recommended for disposal.

Further Explanation:

The food danger zone is the temperature range between 40°F to 140°F. At these temperatures, bacteria grow more rapidly than at other temperatures. At extremely low and high temperatures, bacteria die, but at the food danger zone, they have the temperature that is most suitable for their growth. Bacteria need food, water, and the proper environmental conditions in order to grow and multiply. At 40°F to 140°F (food danger zone), bacteria multiply quickly, with their number doubling within 20 minutes. At this rate, bacteria levels in the food rise making it unsafe for consumption.

It is recommended, therefore, to keep food at temperatures below or well-above the danger zone. Before serving, cold food must be iced and kept at temperatures equal to or below 40°F and hot food warmed at temperatures above 140°F in warming containers and dishes.

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Keywords: food danger zone, food safety

Brown-eye genes are dominant over blue-eye genes. what is the best explanation for the fact that all the blue-eye alleles have not disappeared in the human population?

Answers

Dominant genes will come before recessive. Someone with brown-eye and blue-eye genes will have brown eye since it's the dominant one. So sometimes blue -eye allele is hidden because it's not express, but not always.

People with blue eyes must have all blue-eye allele. Therefore, if there are people with blue eyes you can conclude that blue-eye allele has not disappeared in the human population.

If there is no factor that intervenes with population breeding, Hardy-Weinberg equilibrium will be maintained and that can be used to explain why the all the blue-eye alleles have not disappeared in the human population

Patients are listed in a patient register under a unique number, which makes up the:

Answers

Patients are listed in a patient register under a unique number, which makes up the master patient index.

Patient index number is making the billing and insurance system work more easily. It can also help to differentiate patient with a similar name.

The skin cancer most likely to metastasize is cancer of the melanocytes.

a. True

b. False

Answers

Final answer:

The skin cancer most likely to metastasize is cancer of the melanocytes.

Explanation:

True. Melanoma is the skin cancer most likely to metastasize. Melanoma is a rare but highly aggressive type of skin cancer that affects melanocytes, which are the pigment-producing cells in the skin. If not treated, melanoma can spread to other parts of the body, making it the most serious and deadliest type of skin cancer. Prompt detection and treatment are crucial for improving outcomes.

________ is the term used to describe the crossing over of a tract to the side of the nervous system opposite to where the axons originated.

Answers

Decussation describes the crossing over of a tract to the side of the nervous system opposite to where the axons originated.
This happens in various spinal tracts from body to brain or vice versa, such that we "feel" (sensory) the left side of our body by sensing touch with the right side of our brain, and likewise we "move" (motor) the right side of our body by impulses generated by the left side of our brain.
The term decussation derives from Latin "decussatus", of the word "decussare" which translates into ‘divide crosswise.’
This also relates to the crossing over like that of the figure X, for which the word decussis means. Interesting the Roman numeral for the number 10, which is written "X," derives from this root word into "decem, " for the English number ‘ten.’

Short-term memory is transferred to long-term memory via a process called:

Answers

Mental Repetition

Short-term memory is a necessary step toward the next stage of retention, long-term memory. The transfer of information to long-term memory for more permanent storage can be facilitated or improved by mental repetition of the information or, even more effectively, by giving it a meaning and associating it with other previously acquired knowledge. Motivation is also a consideration, in that information relating to a subject of strong interest to a person, is more likely to be retained in long-term memory.

A fruit fly is classified as a heterotroph because it is unable to do what

Answers

Fix carbon and use it as energy

The outer layer of bone, composed of dense, irregular, collagenous connective tissue that contains blood vessel s and nerves is called

Answers

I wanna say it's periosteum

Providing energy, insulating body organs, and maintaining body temperature are functions of

Answers

Providing energy, insulating body organs, and maintaining body temperature are functions of fats.

Fat is a lipid material of various types and characteristics, it is found in adipose tissue and in other parts of the body.

In the body, fats contribute to the support of the organs, provide protection against the cold and allow the production of energy.

In addition, they fulfill various vital functions, such as serving as a structure to produce hormones and other substances and facilitate the transport of various vitamins.

Fat provides cushioning in certain areas of the body that serves as elastic mechanical protection against pressure.

Dietary fats, like other nutrients, provide energy: about 9kcal / gram, this is what is called caloric intake or calories.

Therefore, we can conclude that providing energy, insulating body organs, and maintaining body temperature are functions of fats.

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Final answer:

Adipose tissue or body fat handles the functions of providing energy, insulating body organs, and maintaining body temperature. It stores energy in the form of triglycerides, insulates the body to conserve heat and protect underlying organs.

Explanation:

The functions of providing energy, insulating body organs, and maintaining body temperature are primarily fulfilled by adipose tissue, or body fat. Adipose tissue serves as a site for long-term energy storage, where nutrients obtained from food are converted to ATP for short-term use while some are stored as triglycerides for prolonged use. Insulation comes from the form of fur, feathers, or fat in animals. In humans, it's the layer of fat that serves as insulation, keeping our bodies warm by conserving heat.

This adipose tissue, which is found in the hypodermis layer of the skin, also forms a 'cushion' protecting underlying organs from damage. Furthermore, bioenergetics in animals shows that no energy system is 100% efficient, and an animal's metabolism produces waste energy in the form of heat. But thanks to insulation provided by adipose tissue, this heat can be conserved to maintain a fairly constant body temperature.

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The Principle of Original Horizontality is the most Select one:

a. biased method to date rocks.
b. complicated method to date rocks.
c. biased explanation for why sediments form layers.
d. creative explanation for why sediments form layers.

Answers

The correct choice is C. It is a biased explanation of why sediments form layers and how layer formation occurs, this is based on the theory that sediments are deposited horizontally and are flat, and when they have not been Altered remain the same, this definition depends on the stratigraphy theory that defines the behavior of the rocks and how the layers are formed.

Final answer:

The Principle of Original Horizontality states that sediment layers are originally deposited horizontally, and any tilting indicates post-depositional tectonic activity.

Explanation:

The Principle of Original Horizontality is neither biased nor complicated; it is a foundational concept in geology that states layers of sediment are initially deposited in horizontal formations due to gravity. If we observe that sedimentary rock layers are tilted, it suggests that such tilting occurred after the deposition due to tectonic forces altering their orientation. This principle is critical in the relative dating of rocks, allowing geologists to infer that any folds, tilts, or distortions in the strata occurred post-deposition, providing information about the geologic history of the region.

Loamy soil is ideal for gardening because it contains

a-mostly silt with very little clay or sand.
b- mostly clay with very little silt or sand.
c-mostly sand with very little clay or silt.
d- roughly equal parts sand, clay, and silt.

Answers

Loamy soil is ideal for gardening because it contains c-mostly sand with very little clay or silt.

Loamy soil is good for gardening since this soil can retain water and nutrient, but if the water is too much it still can be drained away. The soil contains mostly sand which was bigger particle than silt and clay.

Answer:

it is not C i would try D

Explanation:

Stress ________ activity in the sympathetic division of the autonomic nervous system. stress also _________ activity in the parasympathetic division.

Answers

Increases ; decreases

The sympathetic nervous is responsible for the body's reactions to increased stress levels, known more commonly as the flight or fight response. The parasympathetic swimming pool is vital in resting activities, so stress reduces the activity of the parasympathetic nervous system. 

Which statements are correct concerning the creation model:
a. basic kinds of life forms were created by the direct act of God
b. numbers of all species life forms will increase with time
c. every lower life form can be traced back to the same common ancestry
d. basic life forms continued to reproduce their own kind within limits of variation

Answers

a. basic kinds of life forms were created by the direct act of God this can be argued by believes but just choose (A) to b safe

Answer:

The correct answer is a. basic kinds of life forms were created by the direct act of God.

Explanation:

According to the bible and Christianity, there is a creation model which proves that the earth was created by God and life forms present on earth is also created by god.and the organism living.

Creation model says that god destroyed the early earth because it became corrupt and created one more earth on which we are living now. According to the creation model, the creation of earth was completed in 6 days which were 24 hours long.

So, according to the creation model, the correct statement is a. basic kinds of life forms were created by the direct act of God.

during exhalation the diaphragm

Answers

contracts and moves inward, thereby creating chest cavity space

Which is an example of a physical change?

(a) Wood Rots
(b) a nail rusts
(c) Gasoline ignites
(d) water evaporates

Answers

Gasoline igniting doesnt change physically, wood rotting is just it getting old i guess, i would say d because its from water to vapenation. im really bad in school so im not sure but i decided to answer

the answer is water evaporation because it can still be change back to water

Which type of immunity occurs when antibodies pass from mother to fetus via placenta or from mother to baby via breast milk?

Answers

Naturally acquired passive immunity is a type of immunity that occurs when antibodies pass from mother to fetus via placenta or from mother to baby via breast milk. This occurs when a person (or a baby) is exposed to a live pathogen (which came from the mother), and develop a primary immune response which may then lead to immunological memory. 

Answer:

Antibodies, immunity occurs when a mother breastfeeds her baby.

Explanation:

It was very hard to find this in brainly so i hope this helps you :)

How does the melting of the ice caps affect the amount of salt in the water?

Answers

The melting of the ice caps cause the amount of water in the ocean to keep rising and most of the water in the ocean still remains salty.

Final answer:

Melting glaciers and polar ice caps add fresh water to the ocean, potentially decreasing its salinity. The amount of change in salinity varies regionally and is influenced by various factors, including thermal expansion of water and increased acidity of the seas due to carbon dioxide absorption.

Explanation:

As glaciers and polar ice caps melt due to global warming, a significant amount of fresh water that was previously frozen is added to the oceans. This influx of meltwater can dilute the seawater, potentially decreasing the overall salinity of the ocean. However, because the ocean is vast, the change in salinity is not uniform and can vary in different regions.

Melting sea ice, such as icebergs already floating in the ocean, does not directly contribute to changes in salinity since this ice is already part of the ocean's water volume. In contrast, the melting of land-based ice, such as glaciers, adds fresh water not previously accounted for in the sea level, which can reduce the salinity of the seawater around the melting points.

Meltwater from glaciers, combined with the thermal expansion of seawater as it warms, contributes to the rise in global sea levels. This phenomenon not only affects global circulation patterns by altering the density and thus the buoyancy of seawater but also poses a threat to marine ecosystems and coastal communities through increased acidic seas and changing shorelines.

An eating disorder in which a person eats large amounts and then self-induced vomiting or using laxatives is called

Answers

Bulimia Nervosa is the disorder where a person will eat excess amounts and then purge.

Neurons are surrounded by ________, which guide neural connections and clean up after neurons send messages to one another.

Answers

Final answer:

Neurons are surrounded by glial cells, which play a critical role in guiding and maintaining neural functions and outnumber neurons tenfold. In addition to supporting neurons, glia also modulate communication between nerve cells and can be implicated in brain disorders when mutated.

Explanation:

Neurons are surrounded by glia, which guide neural connections and clean up after neurons send messages to one another. Glial cells, often known as the supporting cells of the nervous system, are crucial for the functioning of neurons. They outnumber neurons by a factor of ten and fulfill multiple roles, including guiding developing neurons, buffering harmful ions and chemicals, and insulating axons with myelin sheaths. Not only do glia perform these important support functions, but recent discoveries suggest that they also contribute to nerve activity response and communication modulation between nerve cells. Dysfunctions in glial cells can lead to severe consequences, such as brain tumors, which are mostly caused by mutations in these cells.

Which structures are found in both animal and plant cells? Check all that apply.

Answers

Both of them have ,Cytoplasm, Ribosomes, Mitochondria, Endoplasmic Reticulum (Smooth and Rough), Golgi Apparatus, Microtubules/ Microfilaments,
Nucleus, and Flagella which is found in some animal and plant cells.


cell membrane

cytoplasm

Golgi body

nucleus

ribosome

vacuole

Why is an untreated acute allergic reaction a life-threatening situation?

Answers

An untreated acute allergic reaction can turn life-threatening as the symptoms can escalate rapidly and be potentially harmful.

An untreated acute allergy can also lead to anaphylaxis. Anaphylaxis is a sudden and serious potentially life-threatening allergic reaction.

Anaphylaxis includes symptoms such as irregular pulse, difficulty swallowing and breathing, hyperventilation, fainting, low blood pressure and shock. It can even result in death.

Therefore, it is very important to treat acute allergic reactions.

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If a dog is identified by scanning the shoulder or neck of the animal, what provides the information about the dog?

Answers

The appropriate response is microchip. It appears that the most widely recognized confusion is that the microchip your pet's vet embedded under the skin of your darling pooch is really a GPS microchip gadget for puppies that can enable you to find your pet on the off chance that it gets lost. Truth be told, microchips for mutts and GPS beacons are two unique alternatives.

A student cuts her hand during her gym class. The next day the cut is red and inflamed. What is the likely cause?

Answers

 Immune response to bacterial toxins 

Her organism is responding to the cut!! 

- Have a good one xx

Answer:

D.) Immune response to bacterial toxins

Explanation:

A change in allele frequencies in a population over a span of generations is

Answers

a change in allele frequencies in a population over a span of generations is called microevolution.

A change in allele frequencies in a population over a span of generations is called microevolution.

What is microevolution?

Microevolution is the change in allele frequencies that occurs within a population over time. This variation is the result of four distinct processes: mutation, selection, gene flow, and genetic drift. When compared to macroevolutionary changes, this change occurs in a relatively short period of time.

Pesticide resistance, herbicide resistance, and antibiotic resistance are all examples of natural selection at work. The enterococci bacteria depicted here have developed resistance to a variety of antibiotics.

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